Hematology
Urology
Blood Bank
Microbiology
Cytology/Histology
Miscellaneous |
PAPANICOLAOU STAINING SOLUTIONS
(EA 36, EA 50, EA 65, and OG 6)
RECOMMENDED PROCEDURE:
Smears are alcohol-ether fixed. Smears are brought to distilled water through alcohols.
Stain 2 - 3 minutes in Harris’ or Mayer’s Hematoxylin. Place in distilled water
for 10 seconds. Rinse gently to prevent cells from being washed off (about 5 to 6 dips).
Wash under running tap water for 6 minutes. Rinse in distilled water and transfer through
graded alcohols; 10 seconds with dipping agitation in 50%, 70%, 80% and 95% alcohol.
Stain in OG 6 solution for 1.5 minutes. Rinse smear dipping for 10 seconds in two
individual changes of ethanol. If mucus is present, stain 1.5 minutes in EA
36, EA 50 or EA 65. In each of 3 baths of 95% alcohol, rinse for 10 seconds. In each
of 2 baths of absolute ethyl alcohol, rinse for 10 seconds. Clear in 3 - 4 changes of
xylene.
NOTE: For smears other than
cervical scrapings, e.g. bronchial aspirates, sputum, pleural and peritoneal fluids, EA 65
is substituted for EA 50 or EA 36.
RESULTS: Following the
complete Papanicolaou method of staining, results are as indicated: nuclei stain blue with
clear, sharp detail; cytoplasm stain varying shades of pink, blue, yellow, green, and
gray.
| # 8000 |
Papanicolaou
Staining Solution EA 36
1 Pack (Two 950 ml Btls.) |
$
58.00 |
| # 8010 |
Papanicolaou
Staining Solution EA 36
1 Gallon (3,800 ml) |
71.00 |
| # 8020 |
Papanicolaou
Staining Solution EA 50
1 Pack (Two 950 ml Btls.) |
58.00 |
| # 8030 |
Papanicolaou
Staining Solution EA 50
1 Gallon (3,800 ml) |
71.00 |
| # 8050 |
Papanicolaou
Staining Solution EA 65
1 Pack (Two 950 ml Btls.) |
62.00 |
| # 8060 |
Papanicolaou
Staining Solution EA 65
1 Gallon (3,800 ml) |
71.00 |
| # 8100 |
Papanicolaou
Staining Solution 0G 6
1 Pack (Two 950 ml Btls.) |
58.00 |
| # 8110 |
Papanicolaou
Staining Solution OG 6
1 Gallon (3,800 ml) |
71.00 |
FOR IN VITRO DIAGNOSTIC USE ONLY.
SHORR-SINGLE
DIFFERENTIAL STAIN
A stain for vaginal smears which
provides a sharp differentiation between cornified and non-cornified elements.
RECOMMENDED PROCEDURE:
Fix wet specimen in equal parts of ethanol and ether. Stain for 1 minute. Carry through
70%, 95% and absolute alcohol - 10 dips in each solution. Clear in xylene and mount.
RESULTS:
Cornified elements stain a brilliant orange-red while the non-cornified elements take a
green stain which is deeper in younger cells and paler in those more advanced. The
staining is delicate and clearly reveals cytoplasmic and nuclear detail. WBC’s,
RBC’s, bacteria and sperm are well-differentiated.
| # 8120 |
Shorr-Single
Differential Stain
1 Pack (Two 250 ml Btls.) |
$
119.00 |
| # 8130 |
Shorr-Single
Differential Stain
1 Pack (Two 950 ml Btls.) |
174.00 |
FOR IN VITRO DIAGNOSTIC USE ONLY.
JENNER-GIEMSA
STAIN KIT
(MAY-GRUNWALD GIEMSA METHOD)
Solution I - Jenner Stain (Stock)
Solution II - Giemsa Stain (Stock)
Designed for staining
nuclei, cytoplasm and bacteria in tissue sections.
RECOMMENDED PROCEDURE: Working
Jenner Solution: Mix 25 ml of Sol. I with 25 ml of distilled water.
Working Giemsa Solution: Mix 50 drops of Sol. II with 50 ml. of
distilled water. NOTE: This solution must be made fresh. Do not re-use.
Prior to staining, specimen should be well-fixed, deparaffinized and cleared. Rinse in
distilled water, 2 changes, then methanol, 2 changes at 3 minutes each. Stain in working
Sol. I for 6 minutes. Stain in working Sol. II
for 45 minutes. Handle each slide individually in this and subsequent steps. Differentiate
in 1% glacial acetic acid, then check microscopically. Rinse in distilled water. Dehydrate
in 95% ethanol, absolute ethanol and clear with xylene, 2 changes each.
RESULTS: Nuclei-blue;
cytoplasm-pink to rose; bacteria-blue.
| # 8150 |
Jenner-Giemsa
Stain Kit
1 Kit (100 ml Ea. Sol.) |
$
119.00 |
| # 8151 |
Jenner
Stain (Stock)
1 Btl. (100 ml) |
62.00 |
| # 8152 |
Jenner
Stain (Stock)
1 Pack (Two 100 ml Btls.) |
108.00 |
| # 8153 |
Giemsa
Stain (Stock)
1 Btl. (100 ml) |
62.00 |
| # 8154 |
Giemsa
Stain (Stock)
1 Pack (Two 100 ml Btls.) |
108.00 |
FOR IN VITRO DIAGNOSTIC USE ONLY.
GILL-HEMATOXYLIN
STAIN KIT
Gill-Hematoxylin, 0.2% (Modified), 0.4%
or 0.6%
Solution II - Scott’s Tap Water Substitute
Solution III - Ethanol (95%)
An excellent nuclear stain employing
a half-oxidized hematoxylin solution. (Gill, 1974) Gill Hematoxylin is a high contrast
stain and a progressive hematoxylin which enchances the use of counterstains. To achieve
desired stain characteristics in different specimens, the stain is available in 3
concentrations at 0.2% w/v, (Modified), 0.4% w/v, and 0.6% w/v. The modified solution at
0.2% may be preferred in cytology, however, by increasing the time (10 minutes) it can be
used in histology. Gill-Hematoxylin at 0.4% is recommended for histology, however, a
shorter stain time renders this solution suitable for cytologic use. For comparable
results to those obtained with Harris’ Hematoxylin, Gill-Hematoxylin at 0.6% is
recommended. An essential part of this stain sequence is the use of a blueing agent. We
recommend Scott’s Tap Water Substitute which ensures a controlled pH and aids in cell
retention on slides.
RECOMMENDED PROCEDURE: Gill-Hematoxylin
(Modified): Fix in Sol. III for 15 minutes. Water - 2 x (10 dips each).
Gill-Hematoxylin (Modified) - 2 minutes. Water - 2 x (10 dips each). Sol. II - 1 minute.
Water - 2 x (10 dips each). Sol. III - 2 x (10 dips each). Continue with preferred
staining method. Gill-Hematoxylin 0.4 % or 0.6 %: Xylene - 3 x (2
minutes each). Absolute Ethanol - 3 x (30 seconds each). Water - 2 x (10 dips each). Gill
Hematoxylin 0.4% or 0.6% - 3 minutes. Water - 2 x (10 dips each). Sol. II - 2 minutes.
Water - 2 x (10 dips each). Continue with preferred staining method.
RESULTS: Nuclear chromatin
stains blue to blue-black; cytoplasm faintly tinted.
| # 8400 |
Gill-Hematoxylin
Stain Kit (Modified)
1 Kit (950 ml Ea. Sol.) |
$
99.00 |
| # 8401 |
Gill-Hematoxylin
Stain Kit (0.4%)
1 Kit (950 ml Ea. Sol.) |
102.00 |
| # 8402 |
Gill-Hematoxylin
Stain Kit (0.6%)
1 Kit (950 ml Ea. Sol.) |
115.00 |
| # 8411 |
Gill-Hematoxylin
(Modified)
1 Btl. (950 ml) |
67.00 |
| # 8412 |
Gill-Hematoxylin
(Modified)
1 Kit (Two 950 ml Btls.) |
103.00 |
| # 8413 |
Gill-Hematoxylin
(Modified)
1 Gallon (3,800 ml) |
159.00 |
| # 8421 |
Gill-Hematoxylin
(0.4%)
1 Btl. (950 ml) |
91.00 |
|
# 8422 |
Gill-Hematoxylin
(0.4%)
1 Pack (Two 950 ml Btls.) |
127.00 |
| # 8423 |
Gill-Hematoxylin
(0.4%)
1 Gallon (3,800 ml) |
150.00 |
| # 8431 |
Gill-Hematoxylin
(0.6%)
1 Btl. (950ml) |
92.00 |
| # 8432 |
Gill-Hematoxylin
(0.6%)
1 Pack (Two 950 ml Btls.) |
135.00 |
| # 8433 |
Gill-Hematoxylin
(0.6%)
1 Gallon (3,800 ml) |
182.00 |
| # 8441 |
Sol.
II - Scott’s Tap Water Substitute
1 Btl. (950 ml) |
43.00 |
| # 8442 |
Sol.
II - Scott’s Tap Water Substitute
1 Pack (Two 950 ml Btls.) |
61.00 |
| # 8443 |
Sol.
II - Scott’s Tap Water Substitute
1 Gallon (3,800 ml) |
87.00 |
| # 8451 |
Sol.
III - Ethanol (95%)
1 Btl. (950 ml) |
38.00 |
| # 8452 |
Sol.
III - Ethanol (95%)
1 Pack (Two 950 ml Btls.) |
58.00 |
| # 8453 |
Sol.
III - Ethanol (95%)
1 Gallon (3,800 ml) |
87.00 |
FOR IN VITRO DIAGNOSTIC USE ONLY.
HARRIS’ ALUM
HEMATOXYLIN STAIN
(MERCURY FREE)
A regressive
stain used in demonstrating nuclear chromatin. Stain time is usually 4 - 8 minutes;
however, nature of the tissue and depth of staining may require a time increase to 10 - 15
minutes.
RESULTS: Nuclei stain
brownish-black, cytoplasm stain pale pink.
| # 8490 |
Harris’
Alum Hematoxylin Stain (Mercury Free)
1 Btl. (250 ml) |
$
73.00 |
| # 8491 |
Harris’
Alum Hematoxylin Stain (Mercury Free)
1 Btl. (950 ml) |
91.00 |
| # 8492 |
Harris’
Alum Hematoxylin Stain (Mercury Free)
1 Pack (Two 950 ml Btls.) |
135.00 |
| # 8493 |
Harris’
Alum Hematoxylin Stain (Mercury Free)
1 Gallon (3,800 ml) |
173.00 |
FOR IN VITRO DIAGNOSTIC USE ONLY.
HARRIS’ ALUM
HEMATOXYLIN STAIN
(MERCURY FREE WITH GLACIAL ACETIC ACID)
A regressive
stain used in demonstrating nuclear chromatin. Stain time is usually 4 - 8 minutes;
however, nature of the tissue and depth of staining may require a time increase to 10 - 15
minutes.
RESULTS: Nuclei stain
brownish-black, cytoplasm stain pale pink. Nuclei stain
brownish-black, cytoplasm stain pale pink.
| # 8494 |
Harris’
Alum Hematoxylin Stain (Mercury Free with Glacial Acetic Acid)
1 Btl. (250 ml) |
$
77.00 |
| # 8495 |
Harris’
Alum Hematoxylin Stain (Mercury Free with Glacial Acetic Acid)
1 Btl. (950 ml) |
95.00 |
| # 8496 |
Harris’
Alum Hematoxylin Stain (Mercury Free with Glacial Acetic Acid)
1 Pack (Two 950 ml Btls.) |
161.00 |
| # 8497 |
Harris’
Alum Hematoxylin Stain (Mercury Free with Glacial Acetic Acid)
1 Gallon (3,800 ml) |
206.00 |
FOR IN VITRO DIAGNOSTIC USE ONLY.
HARRIS’ ALUM
HEMATOXYLIN STAIN
(AVAILABLE WITH OR WITHOUT GLACIAL ACETIC ACID)
A regressive
stain used in demonstrating nuclear chromatin. Stain time is usually 4 - 8 minutes;
however, nature of the tissue and depth of staining may require a time increase to 10 - 15
minutes.
RESULTS: Nuclei stain
brownish-black, cyptoplasm stain pale pink.
| # 8500 |
Harris’
Alum Hematoxylin Stain
1 Btl. (250 ml) |
$
50.00 |
| # 8501 |
Harris’
Alum Hematoxylin Stain
1 Btl. (950 ml) |
70.00 |
# 8502 |
Harris’
Alum Hematoxylin Stain
1 Pack (Two 950 ml Btls.) |
99.00 |
# 8503 |
Harris’
Alum Hematoxylin Stain
1 Gallon (3,800 ml) |
132.00 |
#
8404 |
Harris’
Alum Hematoxylin Stain with Glacial Acetic Acid
1 Btl. (475 ml) |
68.00 |
# 8510 |
Harris’
Alum Hematoxylin Stain with Glacial Acetic Acid
1 Pack (Two 950 ml Btls.) |
124.00 |
# 8511 |
Harris’
Alum Hematoxylin Stain with Glacial Acetic Acid
1 Gallon (3,800 ml) |
159.00 |
This solution is made from certified
dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
MAYER’S ACID ALUM
HEMATOXYLIN STAIN
Stain may be used either
progressively or regressively. Excellent as a counterstain and for diagnostic quick
sections. Recommended staining time is 4 - 8 minutes depending upon desired stain
intensity.
| # 8550 |
Mayer’s
Acid Alum Hematoxylin Stain
1 Btl. (250 ml) |
$
62.00 |
# 8551 |
Mayer’s
Acid Alum Hematoxylin Stain
1 Btl. (950 ml) |
71.00 |
| # 8552 |
Mayer’s
Acid Alum Hematoxylin Stain
1 Pack (Two 950 ml Btls.) |
118.00 |
| # 8553 |
Mayer’s
Acid Alum Hematoxylin Stain
1 Gallon (3,800 ml) |
145.00 |
This solution is made from certified dye.
FOR IN VITRO DIAGNOSTIC USE ONLY.
WEIGERT’S IRON
HEMATOXYLIN STAIN KIT
Solution I - Weigert’s Hematoxylin
Solution II - Weigert’s Iron Chloride
Working Solution:
Mix equal parts of Sol. I and Sol. II. Recommended staining time is 2 - 5 minutes.
RESULTS: Nuclei - black;
connective tissue - red; cytoplasm - yellowish.
| # 8600 |
Weigert’s
Iron Hematoxylin Stain Kit
1 Kit (250 ml Ea. Sol.) |
$
98.00 |
| # 8601 |
Weigert’s
Iron Hematoxylin Stain Kit
1 Kit (950 ml Ea. Sol.) |
157.00 |
| # 8602 |
Weigert’s
Iron Hematoxylin Stain Kit
1 Kit (3,800 ml Ea. Sol.) |
225.00 |
These solutions are made from certified
dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
MALLORY’S ACID HEMATOXYLIN
STAIN
(PHOSPHOTUNGSTIC ACID)
A routine stain
method for the nervous system because of its ability to demonstrate abnormal or diseased
astrocytes. Useful in showing mitochondria, striations of cardiac and voluntary muscle,
and as a counterstain for the Periodic Acid-Schiff (PAS) technique. Recommended staining
time is 12 - 24 hours.
RESULTS: Nuclei, mitochondria,
fibrin, fibroglia, microglia, and the contractile portions of striated (voluntary) and
heart muscle stain blue. Collagen, reticulum, elastin, the ground substances of cartilage
and bone stain varying shades of yellow to brownish-red.
| # 8650 |
Mallory’s
Acid Hematoxylin Stain
1 Btl. (250 ml) |
$
74.00 |
# 8651 |
Mallory’s
Acid Hematoxylin Stain
1 Btl. (950 ml) |
99.00 |
| # 8652 |
Mallory’s
Acid Hematoxylin Stain
1 Pack (Two 950 ml Btls.) |
157.00 |
| # 8653 |
Mallory’s
Acid Hematoxylin Stain
1 Gallon (3,800 ml) |
220.00 |
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
HEIDENHAIN’S
IRON HEMATOXYLIN STAIN KIT
Solution I - Ammonio-Ferric Alum
Solution II - 0.5% Aqueous Hematoxylin
A histologic stain composed of ferric
salts which ripen and oxidize hematoxylin rapidly, eliminating artificial or natural
ripening. Solutions made with iron salts are useful for dense regressive staining.
Differentiation can be accurately controlled, and the staining is consistent.
Working Solution:
Dilute Sol. II with an equal quantity of distilled water. Recommended staining time is 1 -
36 hours followed by differentiation in Sol. I.
RESULTS: Chromatin, nucleoli,
mitochondria, centrioles and certain parts of striated muscle fibers are stained black.
Other tissue elements are colored by the contrast stain used.
| # 8700 |
Heidenhain’s
Iron Hematoxylin Stain Kit
1 Kit (250 ml Ea. Sol.) |
$
88.00 |
# 8701 |
Heidenhain’s
Iron Hematoxylin Stain Kit
1 Kit (950 ml Ea. Sol.) |
127.00 |
| # 8702 |
Heidenhain’s
Iron Hematoxylin Stain Kit
1 Kit (3,800 ml Ea. Sol.) |
228.00 |
These solutions are made from certified
dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
EOSIN Y STAINING
SOLUTIONS
(AQUEOUS OR ALCOHOLIC)
Solutions are
used mainly as background contrast to nuclear stains, i.e., hematoxylin-eosin sequence.
Eosin Y stain depicts the cytoplasmic detail of the cell and the tissue structure.
RECOMMENDED PROCEDURE: Stain
15 seconds to 2 minutes depending upon the depth of counterstain desired.
| # 8900 |
Eosin
Y - 1% Aqueous Solution
1 Btl. (250 ml) |
$
62.00 |
# 8901 |
Eosin
Y - 1% Aqueous Solution
1 Btl. (950 ml) |
87.00 |
| # 8902 |
Eosin
Y - 1% Aqueous Solution
1 Pack (Two 950 ml Btls.) |
124.00 |
| # 8903 |
Eosin
Y - 1% Aqueous Solution
1 Gallon (3,800 ml) |
169.00 |
# 8910 |
Eosin
Y - 1% Alcoholic Solution
1 Btl. (250 ml) |
62.00 |
| # 8911 |
Eosin
Y - 1% Alcoholic Solution
1 Btl. (950 ml) |
94.00 |
| # 8912 |
Eosin
Y - 1% Alcoholic Solution
1 Pack (Two 950 ml Btls.) |
145.00 |
# 8913 |
Eosin Y - 1% Alcoholic
Solution
1 Gallon (3,800 ml) |
186.00 |
#
8914 |
Eosin Y -
5% Alcoholic
Solution
1 Btl. (250 ml) |
65.00 |
These solutions are made from certified
dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
EOSIN-PHLOXINE
SOLUTION
Solution is used
mainly as background contrast to nuclear stains, i.e., hematoxylin-eosin sequence. Stain
depicts the cytoplasmic detail of the cell and the tissue structure.
RECOMMENDED PROCEDURE: Stain
15 seconds to 2 minutes depending upon the depth of counterstain desired.
RESULTS: Cytoplasm - pink
| # 8921 |
Eosin-Phloxine
Solution
1 Btl. (950 ml) |
$
108.00 |
# 8922 |
Eosin-Phloxine
Solution
1 Pack (Two 950 ml Btls.) |
149.00 |
| # 8923 |
Eosin-Phloxine
Solution
1 Gallon (3,800 ml) |
193.00 |
| # 8924 |
Eosin-Phloxine
Solution
1 Cubtainer (19 L) |
491.00 |
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
PHLOXINE B COUNTERSTAIN
(1% or 0.05%)
Solution is used
mainly as background contrast to nuclear stains, i.e., hematoxylin-eosin sequence. Stain
depicts the cytoplasmic detail of the cell and the tissue structure.
RECOMMENDED PROCEDURE: Stain
15 seconds to 2 minutes depending upon the depth of counterstain desired.
RESULTS: Cytoplasm - pink
| # 8930 |
Phloxine
B Counterstain (1%)
1 Btl. (950 ml) |
$
108.00 |
| # 8931 |
Phloxine
B Counterstain (1%)
1 Pack (two 950 ml Btls.) |
149.00 |
| # 8932 |
Phloxine
B Counterstain (1%)
1 Gallon (3,800 ml) |
193.00 |
| # 8935 |
Phloxine B Counterstain
(0.05%)
1 Btl. (950 ml) |
108.00 |
| # 8936 |
Phloxine B
Counterstain (0.05%)
1 Pack (Two 950 ml Btls.) |
149.00 |
| # 8937 |
Phloxine B Counterstain
(0.05%)
1 Gallon (3,800 ml) |
193.00 |
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
GOMORI’S
TRICHROME STAIN
(HISTOLOGICAL STAIN)
This stain demonstrates fine
histological detail of connective tissue, collagen and muscle fibers.
RECOMMENDED PROCEDURE:
Deparaffinize and hydrate to distilled water. Place in Bouin’s solution in oven at
560C for 1 hour. Wash well in running water or until yellow color disappears. Stain nuclei
with Weigert’s Iron Hematoxylin for 10 minutes, wash in water, then apply stain for
15 - 20 minutes. Place in 0.5% acetic water for 2 minutes. Dehydrate in 95% ethanol,
absolute ethanol, and clear in xylene, 2 changes each.
RESULTS: Muscle fibers - red;
collagen - green; nuclei -blue to black.
| # 8990 |
Gomori’s Trichrome Stain
1 Pack (Two 250 ml Btls.) |
$ 145.00 |
# 9000 |
Gomori’s Trichrome Stain
1 Pack (Two 950 ml Btls.) |
219.00 |
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
NUCLEAR FAST RED STAIN
(KERNECHTROT)
May be used as a
general counterstain in the following procedures: Gridley’s reticulum stain,
Snook’s reticulum stain, Lepehne’s method for hemoglobin, Ralph’s method
for hemoglobin, Kossa’s method for calcium, Pizzolato’s method, Puchtler-Sweat
method for basement membranes, Osmium tetroxide method for fat in frozen sections,
Gomori’s method for iron, Mallory’s method for iron, Perl’s method for
iron, Tumbull’s Blue method for hemosiderin, Sudan Black B stain for fat, Alcian Blue
(short method) with and without hyaluronidase, Mallory’s reaction for iron, and
Stain’s bile pigment stain.
RECOMMENDED PROCEDURE: The
staining time depends upon the stain procedure being employed. The time varies from a few
seconds to 5 minutes.
RESULTS: Nuclei - pink to red.
# 9040 |
Nuclear
Fast Red Stain (Kernechtrot)
1 Btl. (250 ml) |
$
112.00 |
FOR IN VITRO DIAGNOSTIC USE ONLY.
POTASSIUM PERMANGANATE (0.5%)
A stain used in histopathology for general
laboratory staining procedures.
#
9045 |
Potassium
Permanganate (0.5%)
1 Pack. (Two 250 ml Btls.) |
$
68.00 |
CONTENTS:
Potassium Permanganate
FOR IN VITRO DIAGNOSTIC USE ONLY.
SCHIFF REAGENT
(FOR PERIODIC ACID-SCHIFF REACTION)
A stain used in
histopathology for the staining of glycogen polysaccharides or in combination with the
Periodic Acid-Schiff reaction.
RECOMMENDED PROCEDURE: Stain
time required in the Schiff Reagent is 10 - 15 minutes using standard PAS method.
RESULTS: Glycogen
polysaccharides (carbohydrates) stain magenta to purple indicating a " PAS
positive".
| # 9060 |
Schiff
Reagent
1 Btl. (250 ml) |
$
94.00 |
# 9061 |
Schiff
Reagent
1 Pack (Two 250 ml Btls.) |
149.00 |
| # 9062 |
Schiff
Reagent
1 Pack (Two 950 ml Btls.) |
211.00 |
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
SUDAN BLACK B
STAIN KIT
(FOR FAT)
Solution I - Sudan Black B
Solution II - Propylene Glycol, 85%
Solution III - Nuclear Fast Red Stain (Kernechtrot)
A staining solution used to stain fat
in frozen sections.
RECOMMENDED PROCEDURE:
Dehydrate sections for 5 - 10 minutes in absolute propylene glycol. Stain for 10 - 20
minutes in Sol. I. Differentiate for 2-3 minutes in Sol. III. Wash in distilled
water and counterstain with Sol. III for a few minutes. Wash well and mount.
RESULTS: Fat - blue-black;
nuclei - red.
| # 9100 |
Sudan
Black B Stain Kit
1 Kit (250 ml Ea.Sol.) |
$
235.00 |
# 9110 |
Solution
I - Sudan Black B
1 Pack (Two 250 ml Btls.) |
145.00 |
| # 9111 |
Solution I - Sudan Black
B
1 Btl. (250 ml) |
85.00 |
| # 9120 |
Solution
II - Propylene Glycol, ( 85%)
1 Pack (Two 250 ml Btls.) |
80.00 |
| # 9121 |
Solution II - Propylene
Glycol, (85%)
1 Btl. (250 ml) |
56.00 |
| # 9131 |
Solution
III - Nuclear Fast Red (Kernechtrot)
1 Btl. (250 ml) |
112.00 |
These solutions are made from certified
dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
GOMORI’S
METHOD FOR IRON IN TISSUE WITH NUCLEAR FAST RED OR SAFRANIN
(PRUSSIAN BLUE REACTION)
Solution I - 20% Hydrochloric Acid
Solution II - 10% Potassium Ferrocyanide
Solution III - 0.1% Aqueous Safranin
Solution IIIB - Nuclear Fast Red (Kernechtrot)
Designed to demonstrate deposits of
hemosiderin (iron) in tissue specimens.
RECOMMENDED PROCEDURE: Working
Solution: Mix just prior to use, 50 ml of Sol. I with 50 ml of Sol. II. Run
control slide. Deparaffinize and hydrate to distilled or deionized water. Cover with
working solution for 20 - 30 minutes. Rinse in distilled or deionized water, counterstain
with Sol. III or IIIB for 3 - 5 minutes, and rinse again. Dehydrate in 95% ethanol,
absolute ethanol - 2 changes, clear in xylene - 2 changes, and mount.
NOTE: Chemically clean glassware and plastic-tipped or paraffin-coated forceps are
suggested.
RESULTS: Iron
pigments - bright blue; nuclei - red; cytoplasm - light pink.
| # 9139 |
Gomori’s Method
for Iron in Tissue with Solution IIIB- Nuclear Fast Red
1 Kit (250 ml Ea. Sol.) |
$
210.00 |
# 9140 |
Gomori’s
Method for Iron in Tissue with Solution IIIA - Safranin
1 Kit (250 ml Ea. Sol.) |
149.00 |
| # 9141 |
Solution I - Hydrochloric
Acid (20%)
1 Btl. (250 ml) |
62.00 |
| # 9142 |
Solution II - Potassium
Ferrocyanide (10%)
1 Btl. (250 ml) |
81.00 |
| # 9143 |
Solution IIIA - Aqueous
Safranin (0.1%)
1 Btl. (250 ml) |
50.00 |
| # 9144 |
Solution IIIB - Nuclear
Fast Red
1 Btl. (250 ml) |
112.00 |
C ONTENTS: HCl, Potassium Ferrocyanide, Safranin, Nuclear
Fast Red, Aluminum Sulfate
These solutions are made from certified
dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
STAT STAIN FOR
FROZEN SECTIONS
(TOLUIDINE BLUE-BASIC FUCHSIN)
A polychromatic
solution designed for the rapid staining of frozen sections.
RECOMMENDED PROCEDURE: Float
section onto slide and cover with stain. Immediately rinse slide with tap water.
Dehydrate, clear and mount.
RESULTS: Blue nuclei against a
red cytoplasm.
# 9150 |
Stat
Stain for Frozen Sections
1 Btl. (100 ml) |
$
83.00 |
# 9151 |
Stat
Stain for Frozen Sections
1 Pack (Two 100 ml Btls.) |
132.00 |
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
MAYER’S
MUCICARMINE STAIN KIT
(FOR CRYPTOCOCCUS)
Solution I - (Solution A -
Weigert’s Hematoxylin, Solution B - Weigert’s Iron Chloride)
Solution II - Mayer’s Mucicarmine Stain
Solution III - Metanil Yellow Stain
RECOMMENDED
PROCEDURE: Working Solution I: Mix equal parts of Sol. A with Sol. B. Working
Solution II: Dilute 1 part Sol. II with 4 parts tap water or less for greater intensity in
staining. Deparaffinize and hydrate to distilled water. Apply Working Sol. I - 1-7
minutes. Wash in running water - 10 minutes. Stain in Working Sol. II - 1 hour. Rinse
quickly in distilled water. Stain in Sol. III - 1 minute and rinse again. Dehydrate and
clear in xylene, 2 changes each. NOTE: Carminophilic
properties will be obscured if overstained with Working Sol. I.
RESULTS: Capsule of Cryptococcus
and mucin stain deep rose to red; nuclei stain black.
# 9155 |
Mayer’s
Mucicarmine Stain Kit
1 Kit (250 ml Ea. Sol.) |
$
149.00 |
CONTENTS:
Ethanol, Hematoxylin, Ferric Chloride, HCl, Carmine,
Aluminum Chloride, Metanil Yellow, Acetic Acid
These
solutions are made from certified dyes (when applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
MAYER’S
MUCICARMINE STAIN
A stain designed
to show mucin derived from epithelial cells (bronchi and colon).
RECOMMENDED PROCEDURE: Stain
may be used straight or diluted at 1:4 - 1:10 with distilled water or 50% to 70% alcohol.
The alcoholic solution is preferable. Stain sections for 15 minutes or longer. Wash
quickly in distilled water. Dehydrate, clear and mount.
RESULTS: Mucin stains red.
# 9160 |
Mayer’s
Mucicarmine Stain
1 Btl. (100 ml) |
$
83.00 |
# 9161 |
Mayer’s
Mucicarmine Stain
1 Pack (Two 100 ml Btls.) |
132.00 |
# 9162 |
Mayer’s
Mucicarmine Stain
1 Pack (Two 250 ml Btls.) |
179.00 |
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
BENNHOLD’S
CONGO RED STAIN KIT
(FOR AMYLOID)
Solution I - Congo Red
Solution II - Lithium Carbonate (Saturated Aqueous)
Solution III - Ethanol (80%)
Solution IV - Harris’ Alum Hematoxylin
A simple and reliable procedure which provides a permanent
stain for amyloid.
RECOMMENDED PROCEDURE: Fix in absolute alcohol
or 10% formalin (Product # 9700). Stain in Sol. I for 10 - 20 minutes or longer if a
deeper color is desired. Dip for 15 seconds in Sol. II. Decolorize with Sol. III until
stain no longer washes out. Wash in running water for 15 minutes. Counterstain 1 - 8
minutes in Sol. IV. Wash in tap water, dehydrate, clear and mount.
RESULTS: Amyloid - red; nuclei - blue; hyalin
and colloid - unstained.
| # 9200 |
Bennhold’s Congo
Red Stain Kit
1 Kit (250 ml Ea. Sol.) |
$
197.00 |
# 9210 |
Solution I - Congo Red
1 Pack (Two 250 ml Btls.) |
108.00 |
# 9220 |
Solution II -
Lithium Carbonate (Saturated Aqueous)
1 Pack (Two 250 ml Btls.) |
62.00 |
| # 9230 |
Solution III - Ethanol
(80%)
1 Pack (Two 250 ml Btls.) |
58.00 |
# 9240 |
Solution IV -
Harris’ Alum Hematoxylin
1 Pack (Two 250 ml Btls.) |
104.00 |
These solutions are made from certified dyes (when
applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
VERHOEFF’S
ELASTIC TISSUE STAIN KIT
Solution I - Alcoholic Hematoxylin
Solution II - Aqueous Ferric Chloride (10%)
Solution III - Iodine
Solution IV - Aqueous Ferric Chloride (2%)
Solution V - Aqueous Phloxine (0.5%)
Used to stain elastic fibers, nuclei and collagen.
RECOMMENDED PROCEDURE: Working
Solution: In the following order, mix 20 ml of Sol. I + 8 ml of Sol. II + 8 ml
of Sol. III. NOTE: This staining solution should,be used within 24 hours.
Immerse sections in working solution for 15 minutes to 1 hour until perfectly black.
Differentiate in Sol. IV for a few minutes. Examine sections in water under low
magnification to observe the stages in differentiation. If the differentiation has been
carried too far, (provided they have not been treated with alcohol) the sections may be
restained. Wash in water. Place in 95% alcohol to remove iodine and leave sections in tap
water 5 minutes or longer. Counterstain 3 - 4 minutes
in Sol. V. Clear in xylene and mount.
RESULTS: Elastic fibers - intensely
blue-black; nuclei - blue to black; collagen - red.
| # 9250 |
Verhoeff’s
Elastic Tissue Stain Kit
1 Kit (250 ml Ea. Sol.) |
$
172.00 |
# 9260 |
Verhoeff’s
Elastic Tissue Stain Kit
1 Kit (950 ml Ea. Sol.) |
302.00 |
# 9270 |
Solution I - Alcoholic
Hematoxylin
1 Pack (Two 950 ml Btls.) |
146.00 |
| # 9280 |
Solution II - Aqueous
Ferric Chloride (10%)
1 Pack (Two 950 ml Btls.) |
108.00 |
# 9290 |
Solution III - Iodine
1 Pack (Two 950 ml Btls.) |
119.00 |
| # 9300 |
Solution IV - Aqueous
Ferric Chloride (2%)
1 Pack (Two 950 ml Btls.) |
94.00 |
# 9310 |
Solution V -
Aqueous Phloxine (0.5%)
1 Pack (Two 950 ml Btls.) |
108.00 |
These solutions are made from certified dyes (when
applicable).
FOR IN VITRO DIAGNOSTIC USE ONLY.
MALLORY’S ANILINE
BLUE COLLAGEN STAIN KIT
Solution I - Aniline Blue
Solution II - 0.5% Acid Fuchsin
Demonstrates collagenous fibrils,
cartilage, bone, amyloid, and nuclei.
RECOMMENDED PROCEDURE: Sections should be
Zenker-fixed and procedure for removal of pigments and precipitates should be followed
prior to staining. Stain with Sol. II for 1 - 5
minutes or longer. (Omit Sol. II to highlight collagenous fibrils). Transfer directly to
Sol. I without washing in water. Stain for 20 - 60 minutes or longer. Dehydrate, clear and
mount. For celloidin sections, shorten staining time, decolorize, dehydrate in 95% ethanol
and clear by the blotting paper xylol method or in terpineol and mount in balsam.
RESULTS: Ground substances of cartilage and
bone, mucus, amyloid - varying shades of blue; nuclei, fibroglia, myoglia and neuroglia
fibrils, axis cylinders and fibrin -red; erythrocytes and myelin - yellow; elastic fibrils
- pale pink, pale yellow or unstained.
| #
9330 |
Mallory’s
Aniline Blue Collagen Stain Kit
1 Kit (250 ml Ea. Sol.) |
$
132.00 |
# 9340 |
Mallory’s
Aniline Blue Collagen Stain Kit
1 Kit (950 ml Ea. Sol.) |
194.00 |
# 9350 |
Solution
I - Aniline Blue
1 Pack (Two 950 ml Btls.) |
206.00 |
| # 9360 |
Solution
II - 0.5 % Acid Fuchsin
1 Pack (Two 950 ml Btls.) |
149.00 |
These solutions are made from certified
dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
BISMARCK BROWN WITH
METHYL GREEN STAIN KIT
Solution I - 1% Aqueous Bismarck Brown
Solution II - 0.5% Aqueous Methyl Green
RECOMMENDED
PROCEDURE: Specimens - intestinal (calciform cells), tracheal and embryonic
tissue. Stain in Sol. I for 5 - 10 minutes. Rinse in 95% Ethanol. Transfer to
Sol. II until the slide appears dark green. Dehydrate, clear and mount
in balsam.
RESULTS: Cartilage - dark
brown; mucin - light brown; nuclei of all the cells - green.
| # 9370 |
Bismarck
Brown with Methyl Green Stain Kit
1 Kit (250 ml Ea. Sol.) |
$
132.00 |
# 9380 |
Bismarck
Brown with Methyl Green Stain Kit
1 Kit (950 ml Ea. Sol.) |
218.00 |
# 9390 |
Solution
I - 1% Aqueous Bismarck Brown
1 Pack (Two 950 ml Btls.) |
182.00 |
| # 9400 |
Solution
II - Aqueous Methyl Green (0.5%)
1 Pack (Two 950 ml Btls.) |
182.00 |
These solutions are made from certified
dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
METHYL GREEN WITH PYRONIN
STAIN
Unna Pappenheim
Method modified by Saathof for bacteria, DNA and RNA, and plasma cells.
RECOMMENDED PROCEDURE: Bacteria:
Fixative - absolute alcohol or Carnoy’s fluid. Stain 2 - 4 minutes (up to 30 minutes
if necessary). Wash in distilled water. Dehydrate quickly in absolute alcohol or acetone.
Clear in xylol and mount in balsam. Celloidin sections are cleared in terpineol and
mounted in balsam.
RESULTS: Bacteria - bright red;
chromatin - green; all other structures stain varying shades of red.
RECOMMENDED PROCEDURE: DNA and
RNA: Fixative - Clarke and Camoy’s fluid. Stain 10 minutes. Rinse twice in
distilled water briefly. Blot with smooth filter paper.
Differentiation Solution: Mix
30 ml Tertiary Butyl Alcohol with 10 ml 100% Alcohol. Dehydrate in a fresh change of the
differentiating solution for 1 - 2 minutes. (Slides may be left in solution for 4 - 5
minutes) Clear in two changes of xylene for 10 minutes each.
RESULTS: DNA - blue green; RNA
- bright red; remainder of tissue - pale pink.
RECOMMENDED PROCEDURE: Plasma
cells: Fixative - absolute alcohol or Camoy’s fluid. Stain 20 minutes.
Rinse rapidly in water. Differentiate and dehydrate rapidly in absolute alcohol. Clear in
xylol.
RESULTS: Nuclei - green;
cytoplasm - deep red.
| # 9410 |
Methyl
Green with Pyronin Stain
1 Btl. (250 ml) |
$
108.00 |
# 9411 |
Methyl
Green with Pyronin Stain
1 Pack (Two 250 ml Btls.) |
145.00 |
# 9412 |
Methyl
Green with Pyronin Stain
1 Pack (Two 950 ml Btls.) |
259.00 |
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
NILE BLUE A STAIN
(BASIC BLUE 12; NILE BLUE SULFATE; NIBLAU BX)
A stain used in
histology for the differentiation of melanins and lipofuscins.
RECOMMENDED PROCEDURE:
Deparaffinize and hydrate to distilled water. Stain 20 minutes. Wash 10 - 20 minutes in
running water. Mount in glycerol gelatin.
RESULTS: Lipofuscins - dark
blue or green - blue; melanins - dark green. Cytoplasms: muscle - pale green, red
corpuscles - greenish yellow to greenish blue, myelin - green to deep blue, nuclei - not
stained.
# 9440 |
Nile
Blue A Stain
1 Btl. (250 ml) |
$
108.00 |
# 9441 |
Nile
Blue A Stain
1 Pack (Two 250 ml Btls.) |
157.00 |
This solution is made from certified dyes.
FOR IN VITRO DIAGNOSTIC USE ONLY.
HERXHEIMER’S
SUDAN IV STAIN KIT
(FOR FAT)
Solution I - Sudan IV (Scarlet Red)
Solution II - Ethanol (70%)
Solution III - Mayer’s Acid Alum Hematoxylin
Sudan IV is more soluble in fat than in
alcohol allowing fat to absorb the stain.
RECOMMENDED PROCEDURE: NOTE:
Keep Sol. I tightly stoppered since the evaporation of the alcohol and acetone
causes precipitation of the stain. Dip for an instant in Sol. II. Stain in Sol. I for 2 -
5 minutes. Wash quickly in Sol. II and transfer to distilled water. Counterstain in Sol.
III. Wash thoroughly in water and mount in glycerin.
RESULTS: Nuclei - blue; fat -
orange to red; normal myelin and fatty acids - unstained.
# 9460 |
Herxheimer’s
Sudan IV Stain Kit
1 Kit (250 ml Ea. Sol.) |
$
204.00 |
# 9470 |
Solution
I - Sudan IV (Scarlet Red)
1 Pack (Two 250 ml Btls.) |
119.00 |
# 9480 |
Solution
II - Ethanol (70%)
1 Pack (Two 250 ml Btls.) |
50.00 |
# 9490 |
Solution
III - Mayer’s Acid Alum Hematoxylin
1 Pack (Two 250 ml Btls.) |
119.00 |
|
