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| # 5996 | Methanol
Fixative |
$ 43.00 |
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| # 5997 | Methanol
Fixative |
48.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Hematology Urology Blood Bank Microbiology Cytology/Histology Miscellaneous |
WRIGHT, WRIGHT GIEMSA AND MAY-GRUNWALD GIEMSA STAINS NOTE: Due to the variations in the oxidation of a methylene blue stain, our Wright, Wright Giemsa and May-Grunwald Giemsa stain products are offered with a matched buffer solution of specific pH in order to obtain consistent and uniform staining characteristics from lot to lot. As a result, we recommend our matched buffer solution be used for obtaining optimum results. However, stain and buffer are available separately.H-PACK* STAIN PACKS H-PACK* II STAIN PACK These stain packs are designed to eliminate problems with precipitate, artifacts, drying of slides, and overstaining which causes abnormal staining characteristics of all cellular elements, i.e., false toxic granulation. One adjustment should be all that is necessary to obtain desired color and intensity. Buffer solution compliments individual stain lots to allow consistent staining characteristics. Normal and abnormal WBC’s and platelets are vividly demonstrated. Bacteria within leukocytes can be clearly observed. RBC staining gives excellent results of normal cellular elements including all abnormalities and inclusion bodies. Parasites, such as malaria, are well-defined. Bone marrow preps can be stained routinely. Freezing or high temperature will not cause change in staining ability. Stain pack is designed to be used at room temperature. SHAKE WELL BEFORE USE. RECOMMENDED USES: H-PACK* & H-PACK*II may be used on all specimens requiring a WBC differential, particularly spinal fluid and nasal smears, and any procedure requiring a Wright counterstain. Other uses include LE preps, parasitic elements such as malaria, thicker buffy coat smears, mature and immature elements of routine bone marrow smears, inclusion bodies such as basophilic stippling, etc. All stain clearly and distinctly. RECOMMENDED PROCEDURE: Prior to inserting stain pack, all cannulas, tubing and platen surfaces should be cleaned with methanol. For optimum results, new tubing should be soaked in methanol. In order to prevent staining inconsistencies or artifacts due to quality of slides or humid conditions affecting the smear, we recommend fixing in methanol. (After fixing, it is not necessary to dry slide prior to insertion into machine.) Crenation of red blood cells can be caused by mechanical changes in preparation of slide prior to staining. H-PACK*: Place stain pack into your instrument with the cut out holes facing forward. Insert cannulas through plastic bottles until flush. NOTE: If cannulas are dull and do not puncture bottles smoothly, use an 18 - 20 gauge needle to puncture bottles prior to inserting cannulas. H-PACK* II: The cannulas are inserted into the appropriate containers of solution. The stain container has two cannulas, one inserted at each end of the container. The buffer and rinse containers have one cannula each. RECOMMENDATION FOR MACHINE SETTINGS: Due to the inconsistency in the pump motors of the Ames HEMA-TEK** Slide Stainers and the constant wear of the pump tubing, we recommend that a daily setting be made in order to obtain consistent results from day to day.H-PACK* WRIGHT, WRIGHT GIEMSA AND MAY-GRUNWALD GIEMSA STAINS: Ames Hema-Tek** and Ames Hema-Tek ** 1000: All pumps should initially be set on maximum by rotating the pumps completely to the right. For a more acid (red) stain, keep buffer and rinse pumps on full and reduce stain pumps by rotating setting to the left. For a more alkaline (blue) stain, keep stain and rinse pumps on full and reduce buffer by rotating setting to the left. Ames Hema-Tek** 2000: All pumps should initially be set to +2. For a more acid (red) stain, adjust only stain pump by rotating setting to 0 or -2. For a more alkaline (blue) stain, adjust only buffer pump by rotating setting to 0 or -2. Due to the unstable characteristics of formulating a stain pack that contains both a Wright, Giemsa or May-Grunwald stains, the presence of some residue on the platen will be seen after use. It is suggested that the platen surface be cleaned at frequent intervals to prevent build-up of stain residue on the platen. H-PACK* II WRIGHT GIEMSA STAIN: Since the H-PACK* II stain requires a shorter staining time, both quantities of solution and time may be decreased. Setting the rinse down to the minimum, stain volume and stain intensity control may be decreased yielding excellent staining quality. NOTE: RINSE SHOULD BE SET ON FULL AT ALL TIMES TO AID IN CLEANING AND DRYING OF SLIDES. TROUBLESHOOTING COMMON STAINING PROBLEMS: Ames HEMA-TEK** Slide Stainers: Change all tubing monthly and flush stain line with methanol. Use 25 gauge needle and syringe and place in stain opening on platen. Remove stain pump tubing and check to determine if methanol is flushing out from rear connector. Clean platen and surrounding wells. Remove all debris and be careful not to bend finger switches. Make sure finger switches are clean and floating freely. Run blank slide through stainer and check pump times. Stain pump should be activated when center of slide is over opening on platen and pump should run for about 8 seconds. Be sure the slide is completely covered with the stain. Buffer pump should be activated when edge of slide is over opening on platen and pump should run for about 45 seconds. Rinse pump should be activated when edge of slide is over opening on platen. This should run for 40 - 45 seconds and pump should remain on full. Adjust finger switches to correct. * Trademark of ENG Scientific, Inc.** Trademark of AMES Co., Elkhart, IL PRECAUTIONS: The solvent for the stain is methanol, which is flammable. Store at room temperature. IN THE EVENT OF COLD WEATHER, allow solution to return to room temperature and SHAKE WELL BEFORE USE.
FOR IN VITRO DIAGNOSTIC USE ONLY. TRAXSTAIN* RECOMMENDED PROCEDURE FOR MIDAS, HEMASTAINER, AND CARL ZEISS HMS SLIDE STAINERS: Fill Station 1 with Traxstain Fixative. Fill Station 2 with Traxstain (Wright, Wright Giemsa or May- Grunwald Giemsa stain). Fill station 3 by mixing 80 ml of Traxstain with 420 ml of Buffer. Station 4 is a rinse station as provided for the instrument. Fill station 5 with Traxstain Buffer. RECOMMENDED PROCEDURE FOR MIDAS II, AND SAKURA RSG-61 SLIDE STAINERS: Fill Station 1 with Traxstain Fixative. Fill Station 2 with Traxstain (Wright, Wright Giemsa or May-Grunwald Giemsa Stain). Fill station 3 by mixing 50 ml of Traxstain with 250 ml of Buffer. Station 4 is a rinse station as provided for the instrument. Fill station 5 with Traxstain Buffer.
--------------SUGGESTED
TIMES-------------
NOTE: These are median times and may be adjusted for desired intensity, keeping a ratio of 1:2 for Station 2 and 3. The rinse water must be within the range of pH 6.5 to 7.2. In order to obtain proper staining results, we suggest using the minimum time setting for this station, i.e. 5 seconds. Stain color is adjusted by increasing or decreasing time in Station Number 5 (Traxstain Buffer); less time, more alkaline (blue), more time, more acidic (red). IN THE EVENT OF COLD WEATHER, allow solution to return to room temperature and SHAKE WELL BEFORE USE.
CONTENTS: Wright Stain, Giemsa Stain, May-Grunwald Stain, Methanol, Buffers, Stabilizers. These solutions are made from certified
dyes (when applicable). SYSTAIN* RECOMMENDED PROCEDURE: Fill stain reservoir with Wright or Wright Giemsa Systain. Fill buffer and rinse reservoirs with Systain Buffer. NOTE: Do not use deionized water in rinse reservoir. Approximately five blank slides should be run to allow for priming of the system.
SYSTAIN* PROTOCOL Stain 1 Staining Time
2
Minutes 4 Minutes Stain 1 Dilution Time 4-8 Minutes 8-12 Minutes Stain 2 Dilution Time (Not used) 0 Minutes 0 Minutes Dry (Base upon humidity in lab) 5 Minutes 5 Minutes NOTE: These are median times. Stain color is adjusted by increasing or decreasing Stain 1 Staining Time; less time, more alkaline (blue), more time, more acidic (red). IN THE EVENT OF COLD WEATHER, allow solutions to return to room temperature and SHAKE WELL BEFORE USE.
CONTENTS: Wright Stain, Giemsa Stain, Methanol, Buffers, Stabilizers These solutions are made from certified dyes (when
applicable). *Trademark of Eng
Scientific, Inc. QUICK STAIN KIT Solution I - Wright or Wright
Giemsa Stain An alcoholic quick stain technique (fixation not necessary) requiring less than one minute yielding excellent staining results without precipitate. RECOMMENDED PROCEDURE: Pour solutions into three separate coplin jars. Place slide in Sol. I for 10 seconds. DO NOT AGITATE. Place in Sol. II for 10 seconds. DO NOT AGITATE. Place in Sol. III for 10 seconds. DO NOT AGITATE. For adequate rinsing, dip slide in and out of Sol. III approximately 3 - 4 times. Allow to dry. NOTE: Less time in Sol. III provides a more alkaline (blue) stain; increase time for a more acidic (red) stain. IN THE EVENT OF COLD WEATHER, allow solutions to return to room temperature and SHAKE WELL BEFORE USE. * Trademark of Eng Scientific, Inc.
FOR IN VITRO DIAGNOSTIC USE ONLY. STAT-QUICK
STAIN No fixation required with this stain. Utilized in both stat and routine laboratories. Only two solutions are necessary to obtain excellent staining characteristics. Matching buffer solution allows uniform staining of all cellular elements without precipitate or artifactual changes. RECOMMENDED PROCEDURE: Place slide in stain for 20 seconds. DO NOT AGITATE. Place slide in buffer (water, if used, see NOTE) for 20 seconds depending upon color desired. DO NOT AGITATE. For a more alkaline (blue) stain, decrease time in buffer solution. For a more acid (red) stain, increase time in buffer solution. To facilitate adequate cleansing of slide, shake 3 - 4 times in buffer solution. Allow to dry. NOTE: For more intense staining, increase time in stain and buffer solution. We have noted that in some areas the tap water as well as distilled water varies in pH.This will result in improper staining. We recommend that our buffer solution be used to maintain consistency in staining quality. If water is used, check pH of the water to be in the range of 6.7 to under 6.8. STAIN CONTAINER SHOULD ALWAYS BE SHAKEN BEFORE USE, and the stain placed in the staining container should be mixed periodically. IN THE EVENT OF COLD WEATHER, allow solution to return to room temperature and SHAKE WELL BEFORE USE.
FOR IN VITRO DIAGNOSTIC USE ONLY. MANUAL METHOD
STAIN This solution, using a flooding technique, will stain all cellular elements without precipitate or artifactural changes. Staining characteristics are similar to H-PACK*. Buffer is applied immediately after stain, reducing overall staining time. Convenient flip-top cap for 250 ml and 950 ml sizes. IN THE EVENT OF COLD WEATHER, allow solution to return to room temperature and SHAKE WELL BEFORE USE. RECOMMENDED PROCEDURE: We recommend slides be fixed in methanol. Flood slide with stain. Immediately, add equal amount of buffer to fully cover and mix with stain. (Blow gently on slide to adequately mix both buffer and stain.) Stain 5 - 7 minutes depending on color intensity desired. If you desire a more alkaline (blue) stain, decrease staining time. If you prefer a more acid (red) stain, increase staining time. Rinse slide with distilled water and allow to dry before reading.
FOR IN VITRO DIAGNOSTIC USE ONLY. METHANOL FIXATIVE For use as a fixative prior to staining bloody specimen material or blood culture supematant fluid. Methanol preserves the morphology of red blood cells as well as bacteria. RECOMMENDED PROCEDURE: Place material to be stained on a clean glass slide. Flood slide with methanol fixative for 1 minute. Drain without rinsing and allow slide to air dry prior to staining.
CONTENTS: Methanol FOR IN VITRO DIAGNOSTIC USE ONLY. DIP STAIN KIT Solution I - Fixative RECOMMENDED PROCEDURE: Fix slide in Sol. I for 30 seconds. Place in Sol. II for 3 minutes followed by Sol. III for 1 minute. NOTE: The color can be varied by either increasing or decreasing time in Sol. III (Matching Buffer). For a more basic stain (blue), decrease time in Sol. III; for a more acid stain (red), increase time. Dip slide in Sol. IV for 3 - 5 seconds. NOTE: Due to evaporation and absorption of moisture, it may be necessary to increase time in Sol. I (Fixative) to 1 minute, Sol. II (Stain) to 3.5 minutes, Sol. IV (Alcohol Wash) may be increased to 30 seconds. Solutions should be changed after a 24 hour period with cleaning of all reagent containers. Solutions should be replenished during staining period. IN THE EVENT OF COLD WEATHER, allow solutions to return to room temperature and SHAKE WELL BEFORE USE.
CONTENTS: Wright Stain, Giemsa Stain (as indicated), Methanol, Buffers, Stabilizers These solutions are made from certified dyes (when applicable).FOR IN VITRO DIAGNOSTIC USE ONLY. GIEMSA STAIN RECOMMENDED PROCEDURE: For use as a counterstain, dilute stock Giemsa with an equal volume of buffer or distilled water. We suggest a 20 minute staining time. If used as a straight Giemsa stain, increase ratio of stain to buffer for darker staining. NOTE: We recommend a buffer solution within a 6.8 pH range or distilled water with a comparable pH range.
CONTENTS: Giemsa Stain, Methanol This solution is made from certified dyes.FOR IN VITRO DIAGNOSTIC USE ONLY. WOLBACH'S GIEMSA STAIN A stain used to stain bone marrow preparations and to aid in the identification of bacteria, rickettsia, and collagen. RECOMMENDED PROCEDURE: Deparaffinize and hydrate to distilled water. Remove mercuric chloride crystals with iodine and clear with sodium thiosulfate. Wash in running water for 15 minutes. Rinse in distilled water. Working Wolbach's Giemsa solution* overnight. Differentiate in working rosin alcohol solution until sections assume a purplish pink color. Check microscopically. Dehydrate in absolute alcohol then clear in xylene, two changes each. Mount with Permount or Histoclad. *Working Solution: Distilled water - 50 ml, Methanol - 1.5 ml, Wolbach's Giemsa - 1.25 ml.
CONTENTS: Giemsa Stain, Methanol, Glycerin This solution is made from certified dyes.FOR IN VITRO DIAGNOSTIC USE ONLY.
NATT-HERRICK'S
STAIN A solution that stains all white blood cells dark violet distinguishing them from red blood cells. RECOMMENDED PROCEDURE: Draw venous or capillary blood to the 1.0 mark in a white cell pipette. Draw eosinophil solution to the 11.0 mark and mix gently for 30 seconds. NOTE: Prolonged, vigorous shaking will tend to cause rupturing of eosinophils. Allow to stand between 5 - 15 minutes. Shake again, charge chamber and allow cells to settle before counting. 1. Prepare a 1:200 dilution of blood with Natt-Herrick's stain (add 20 ul blood to 4 ml of Natt-Herrick's stain). 2. Mix well, and leave at room temperature for 5 minutes: then fill both sides of a hemocytometer with the stained blood. 3. After 5 minutes, perform a white blood cell count, using 10X objective. That is, count all white blood cells in the 4 large corner squares on both sides of the hemocytometer chamber (the counts within each square should be within 10 percent of each other). Add all 8 counts together and use this total count to calculate.
Total # WBC's X 2000 = # WBC's/ul blood All white blood cells will stain dark violet.
CONTENTS: Sodium chloride, Sodium sulfate, Sodium phosphate, Potassium phosphate, Formalin, Methyl Violet. This solution is made
from certified dyes. EOSINOPHIL DILUTING FLUID AND STAIN Eosinophil counts are useful in determining an increase (eosinophilia) or decrease (eosinopenia) in eosinophilic granulocytes. This technique, used to perform an absolute count, requires a special diluent and/or stain solution. This diluting fluid will lyse the erythrocytes and only eosinophils will stain a bright orange-red. RECOMMENDED PROCEDURE: Draw venous or capillary blood to the 1.0 mark in a white cell pipette. Draw eosinophil solution to the 11.0 mark and mix gently for 30 seconds. NOTE: Prolonged, vigorous shaking will tend to cause rupturing of eosinophils. Allow to stand between 5 - 15 minutes. Shake again, charge chamber and allow cells to settle before counting. Cells Counted x Dilution (10) x
Depth (10) = Total Cells / cmm
CONTENTS: Phloxine, Propylene Glycol, Sodium Carbonate This solution is made
from certified dyes. EOSINOPHIL DILUTING FLUID AND STAIN (FOR VETERINARY USE) A diluting fluid that renders the red blood cells invisible (hemolyzes) and lyses all WBC's except eosinophils. RECOMMENDED PROCEDURE: Draw venous or capillary blood to the 1.0 mark in a white cell pipette. Draw eosinophil solution to the 11.0 mark and mix gently for 30 seconds. NOTE: Prolonged, vigorous shaking will tend to cause rupturing of eosinophils. Allow to stand between 5 - 15 minutes. Shake again, charge chamber and allow cells to settle before counting. 1. Draw venous or capillary blood to the 1.0 mark in a white cell pipette. 2. Draw eosinophil solution to the 11.0 mark and mix gently for 30 seconds. NOTE: Prolonged, vigorous shaking will tend to cause rupturing of eosinophils. 3. Allow to stand between 5 - 15 minutes. 4. Shake again, charge chamber and allow cells to settle before counting.Cells Counted x Dilution (10) x
Depth (10) = Total Cells / cmm
CONTENTS: Phloxine, Propylene Glycol, Sodium Carbonate Phloxine, Propylene Glycol, Sodium Carbonate .This solution is made
from certified dyes. URINARY
SEDIMENT STAIN A quick staining procedure by which a drop of stain solution is directly added to urine sediment. Neutrophilic leukocytes stain violet with red-purple nuclei. "Glitter" cells stain light blue to almost colorless. Squamous vaginal epithelial cells stain pale purple; nucleus stains dark purple. Bladder epithelial cells are either colorless or a pale blue. Hyaline casts stain a delicate pink to rose shade. Granulation stains red violet, or blue in granular casts. WBC, REC, epithelial, and cellular casts are easily recognized by the intense staining characteristics of cellular inclusions. Fatty cells have a bright honeycomb-like structure in a slightly stained matrix. Bacteria stain pink when living and active; dark purple when dead. Yeast cells may stain dark purple or they may not take the stain at all. Trichomonas parasites are either colorless or pale blue. RBC’s stain faintly.
CONTENTS: Crystal Violet, Ammonium Oxalate, Ethanol, Safranin O This solution is made from certified dyes.FOR IN VITRO DIAGNOSTIC USE ONLY. PRUSSIAN
BLUE STAIN KIT Solution I - Potassium
Ferrocyanide (2%) RECOMMENDED PROCEDURE: Working solution: Just prior to use, mix 5 ml of Sol. I with 5 ml of Sol. II. Hemosiderin in urine appears as yellow-brown granules seen either free or within epithelial cells and occasionally in casts. Examine several drops of a centrifuged urine specimen for coarse brown granules. If coarse brown granules are present, resuspend the remainder of the sediment in the working solution and allow to remain for 10 minutes. Centrifuge specimen, discard supematent and examine microscopically. NOTE: The use of chemically clean glassware and plastic-tipped or paraffin-coated forceps is suggested. RESULTS: Coarse granules of hemosiderin stain blue.
CONTENTS: Potassium Ferrocyanide, HCl For in vitro diagnostic use only.MALARIAL
WRIGHT GIEMSA STAIN KIT Solution I - Fixative This stain kit (a modified Wright Giemsa formulation) utilizes an alcohol fixative and stain in combination with a buffer solution (pH range - 6.8 to 7.2). All cellular elements are stained. The use of a thin smear allows for easy visualization of inclusions, extracellular forms and the size of red cells. Their characteristic morphologies are used for differentiation. RECOMMENDED PROCEDURE: Make a thin blood smear. Fix slide in Sol. I from 10 - 30 seconds. Flood slide with Sol. II. Immediately overlay with equal volume of Sol. III. Stain 3 - 6 minutes depending upon color intensity desired. Rinse and allow slide to dry in a vertical position.
CONTENTS: Wright Stain, Giemsa Stain, Methanol, Buffers, Stabilizers These solutions are made from certified dyes (when applicable).FOR IN VITRO DIAGNOSTIC USE ONLY. MALARIAL
QUICK STAIN KIT Solution I - Buffered Stain A combination of water-based buffered stains (pH range = 6.8 to 7.2) whereby the RBC’s are hemolyzed allowing the malarial parasites to be stained. Thick films provide a greater volume of blood and therefore, larger numbers of organisms for examination. However, characteristic definitive morphologic criteria may be more difficult to see. The cytoplasm of white blood cells will stain with eosinophils predominant. RECOMMENDED PROCEDURE: Allow blood film to air dry completely. DO NOT FIX IN ALCOHOL OR HEAT. Place enough of Sol. I and Sol. II into two separate coplin jars. Place unfixed blood smear in Sol. I for 1 - 3 seconds. Remove and rinse under tap water until all stain is removed. Place in Sol. II for 2 seconds. Rinse under tap water 2 - 3 seconds and allow to dry.
CONTENTS: Methylene Blue, Giemsa Stain, Eosin Y, Buffers, Stabilizers These solutions are made from certified dyes (when applicable).FOR IN VITRO DIAGNOSTIC USE ONLY. MAY- GRUNWALD
STAIN KIT Solution I - May-Grunwald Stain RECOMMENDED PROCEDURE: Fix smears for 3 minutes in methanol. Cover slide with Sol. I for 3 minutes. Add an equal amount of distilled water and allow to stand 1 minute. Drain without rinsing. Cover for 12 minutes with dilute Giemsa stain (15 drops of Sol. II + 10 drops of distilled water.) Differentiate in distilled water by agitating for about 5 seconds and checking under microscope. Blot dry and mount. RESULTS: Similar to a Wright Giemsa stain.
CONTENTS: May-Grunwald Stain, Giemsa Stain, Methanol These solutions are made from certified dyes (when applicable).FOR IN VITRO DIAGNOSTIC USE ONLY. BONE
MARROW STAIN KIT FOR IRON Solution I - Potassium Ferrocyanide
(2%) RESULTS: Hemosiderin and ferritin stain blue and is usually reported as either negative or 1+ to 5+. Iron in hemoglobin is unstained.
CONTENTS:
Potassium Ferrocyanide, HCl SIDEROCYTE
STAIN KIT Solution I - Potassium
Ferrocyanide (2%) RESULTS: Siderotic granules stain bright blue.
CONTENTS: Potassium Ferrocyanide, HCl, Safranin O These solutions are made from certified dyes (when applicable).FOR IN VITRO DIAGNOSTIC USE ONLY. SUDAN BLACK B STAIN KIT Solution I - Sudan Black B This technique may be substituted for the benzidine-peroxidase method used for the differential diagnosis of acute granulocytic leukemia, acute lymphocytic and acute myelo-monocytic leukemia. RECOMMENDED PROCEDURE: Working Solution: Prepare as needed by mixing 60 ml of Sol. I with 40 ml of Sol. II. Filter, using suction. Fixation: Place several pieces of filter paper in the bottom of a Coplin jar with screw-top lid. Moisten with 3 - 4 drops of 37% formaldehyde. Place air-dried smears in Coplin jar and cap tightly. Fix for 10 minutes. Wash in running water for 2 minutes. Place smears in working solution for 30 - 60 minutes. Wash in Sol. III for 30 - 60 seconds to remove excess stain. Wash in tap water for 2 minutes. Counterstain in Sol. IV for 10 minutes. Wash in tap water for 5 minutes. RESULTS: Lymphocytes and normoblasts are not stained with Sudan Black B. Normal granulocyte precursors from blast cells onward show increasing sudanophilia corresponding roughly to the granules present. Promyelocytes contain a few sudanophilic granules, while mature polymorphonuclear neutrophils contain large numbers of sudanophilic granules. Auer bodies are intensely sudanophilic.
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