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| Hematology Urology Blood Bank Microbiology Cytology/Histology Miscellaneous |
URINARY SEDIMENT STAIN A quick staining procedure by which a drop of stain solution is directly added to urine sediment. Neutrophilic leukocytes stain violet with red-purple nuclei. "Glitter" cells stain light blue to almost colorless. Squamous vaginal epithelial cells stain pale purple; nucleus stains dark purple. Bladder epithelial cells are either colorless or a pale blue. Hyaline casts stain a delicate pink to rose shade. Granulation stains red violet, or blue in granular casts. WBC, RBC, epithelial, and cellular casts are easily recognized by the intense staining characteristics of cellular inclusions. Fatty cells have a bright honeycomb-like structure in a slightly stained matrix. Bacteria stain pink when living and active; dark purple when dead. Yeast cells may stain dark purple or they may not take the stain at all. Trichomonas parasites are either colorless or pale blue. RBC’s stain faintly.
CONTENTS: Crystal Violet, Ammonium Oxalate, Ethanol, Safranin O This solution is made from certified dyes.FOR IN VITRO DIAGNOSTIC USE ONLY. PRUSSIAN BLUE
STAIN KIT Solution II - 1% Hydrochloric Acid RECOMMENDED PROCEDURE: Working solution: Just prior to use, mix 5 ml of Sol. I with 5 ml of Sol. II. Hemosiderin in urine appears as yellow-brown granules seen either free or within epithelial cells and occasionally in casts. Examine several drops of a centrifuged urine specimen for coarse brown granules. If coarse brown granules are present, resuspend the remainder of the sediment in the working solution and allow to remain for 10 minutes. Centrifuge specimen, discard supernatant and examine microscopically. NOTE: The use of chemically clean glassware and plastic-tipped or paraffin-coated forceps is suggested. RESULTS: Coarse granules of hemosiderin stain blue.
FOR IN VITRO DIAGNOSTIC USE ONLY. FECAL STAIN KIT Solution I - Ethanol 95% Specifically for use in the microscopic examination of fecal material for fatty acids and neutral fats, as well as fecal leukocytes. RECOMMENDED PROCEDURE: Neutral Fats: Place a small aliquot of fecal suspension on a clean glass slide. Mix with 2 drops of Sol. I. Add and mix 2 drops of Sol. II. Coverslip and read microscopically. Fatty Acids: Place a small aliquot of fecal suspension on a clean, glass slide. Mix with 2 drops of Sol. III. Add and mix 2 drops of Sol. II. Coverslip and heat to boiling for a few seconds. Examine microscopically under high power while still warm. Fecal Leukocytes: Place a small aliquot of mucus or liquid stool on a clean, glass slide. Add 2 drops of Sol. IV and mix. Coverslip and allow to stand for 2 - 3 minutes before examining microscopically. NOTE: Fresh material should be used for optimum results. RESULTS: Neutral fats form yellow to pale orange refractile globules. Fatty acids appear as deep orange globules. To determine the presence of fecal leukocytes, examine stained specimen under low power. Differential counts (mononuclear or polymorphonuclear cells only) should be performed under high power.
CONTENTS: Ethanol, Acetic Acid, Sudan IV, Methylene Blue These solutions are made from certified dyes (when applicable).FOR IN VITRO DIAGNOSTIC USE ONLY. FECAL LEUKOCYTE
STAIN Specifically used in the microscopic identification and counting of fecal leukocytes. RECOMMENDED PROCEDURE: Place a small aliquot of mucus or liquid stool on a clean glass slide. Add 2 drops of Loeffler’s methylene blue and mix. Coverslip and allow to stand for 2 - 3 minutes before examining microscopically. NOTE: Fresh material should be used for optimum results. RESULTS: Fecal leukocytes stained with Loeffler’s methylene blue can be visualized under low power. Differential counts (mononuclear or polymorphonuclear cells only) should be performed under high power.
CONTENTS: Ethanol, Methylene Blue This solution is made from certified
dyes. METHYLENE BLUE SOLUTIONS Solutions are used as a counterstain or for wet mount preparation. RECOMMENDED PROCEDURE: A small amount of feces is picked up with an applicator stick and thoroughly mixed with a large drop of methylene blue solution. The mixture is covered with a coverslip and sealed. The preparation should be examined within approximately 30 minutes. RESULTS: Trophozoite nuclei are stained blue with a lighter cytoplasm. Inclusions of the nuclei are also stained. After a time, the organisms become over-stained and are no longer identifiable.
CONTENTS: Methylene Blue These solutions are made from
certified dyes. SUDAN IV STAIN KIT Solution I - Ethanol 95% A simple qualitative screening procedure for the microscopic examination of fat in feces. RECOMMENDED PROCEDURE: Place a small aliquot of fecal suspension on a clean glass slide. Mix with 2 drops of Sol. I. Add and mix 2 drops of Sol. II. Coverslip and read microscopically. RESULTS: Neutral fats stain pale orange to red.
CONTENTS: Sudan IV, Ethanol These solutions are made from certified dyes (when applicable).FOR IN VITRO DIAGNOSTIC USE ONLY. SUDAN IV
(SCARLET RED) STAIN A simple qualitative screening procedure for the microscopic examination of fat in urine. RECOMMENDED PROCEDURE: Place a drop of urine from the surface of a centrifuged specimen or from centrifuged urine sediment on a clean glass slide, making two deposits. Add one drop of Sudan Solution to one drop and coverslip both droplets individuallly. Look at unstained specimen before looking at stained droplet. The staining reaction can take a few minutes.
RESULTS: Fats, whether free, in tubular cells, or in casts are stained red-orange CONTENTS: Sudan IV, Ethanol This solution is made from certified dye. SEMEN A NALYSIS KIT(MODIFIED BLOM'S TECHNIQUE) Solution I - Semen Diluting Fluid This kit consists of a diluting fluid that renders spermatozoa immobile for easy chamber counting; a vitality stain that will easily differentiate between viable and non-viable spermatozoa; and a rapid stain and buffer solution requiring less than 60 seconds for the detection of pus cells (WBC’s, RBC’s, etc.). RECOMMENDED PROCEDURE: Sperm Count: Draw semen up to 0.5 mark (WBC pipette). Dilute to the 11.0 mark with Sol. I. Shake well. Charge hemacytometer and place in humidified chamber to avoid evaporation if unable to read immediately. Count the number of spermatazoa RESULTS: Total Count: 60,000,000 and higher. Vitality Total Count: 60,000,000 and higher. Vitality Stain: Dead, non-viable spermatozoa will stain red, while viable spermatozoa will be unstained (The Blom Technique). Stain For Cells, etc.: Similar to Wright Stain.
CONTENTS: Sodium Bicarbonate, Formalin (Neutral), Eosin, Nigrosin, Wright Stain, Methanol, Buffers Sodium Bicarbonate, Formalin (Neutral), Eosin, Nigrosin, Wright Stain, Methanol, Buffers These solutions are made from certified dyes (when applicable).FOR IN VITRO DIAGNOSTIC USE ONLY. SEMEN ANALYSIS KIT Solution I - Semen Diluting Fluid This kit consists of a diluting fluid that renders spermatozoa immobile for easy chamber counting; a vitality stain that will easily differentiate between viable and non-viable spermatozoa; and a rapid stain and buffer solution requiring less than 60 seconds for the detection of pus cells (WBC’s, RBC’s, etc.). RECOMMENDED PROCEDURE: Sperm Count: Draw semen up to 0.5 mark (WBC pipette). Dilute to the 11.0 mark with Sol. I. Shake well. Charge hemacytometer and place in humidified chamber to avoid evaporation if unable to read immediately. Count the number of spermatazoa in 2 sq. mm and add 5 zeros to obtain the number per ml. Vitality Staining: Mix a drop of semen with 2 drops Sol. IIA. Add 4 drops of Sol. IIB, mix gently and prepare smears. Fix by heat. Staining For White Cells, Red Cells, Spermatozoa Morphology, etc.: Smear a drop of semen on a clean glass slide. Place in Sol. IIIA for 20 seconds, followed by Sol. IIIB for 20 seconds. Sperm Count: Draw semen up to 0.5 mark (WBC pipette). Dilute to the 11.0 mark with Sol. I. Shake well. Charge hemacytometer and place in humidified chamber to avoid evaporation if unable to read immediately. Count the number of spermatazoa in 2 sq. mm and add 5 zeros to obtain the number per ml. Vitality Staining: Mix a drop of semen with 2 drops Sol. IIA. Add 4 drops of Sol. IIB, mix gently and prepare smears. Fix by heat. Staining For White Cells, Red Cells, Spermatozoa Morphology, etc.: Smear a drop of semen on a clean glass slide. Place in Sol. IIIA for 20 seconds, followed by Sol. IIIB for 20 seconds. RESULTS: Total Count: 60,000,000 and higher. Vitality Total Count: 60,000,000 and higher. Vitality Stain: Dead, non-viable spermatozoa will stain red, while viable spermatozoa will be unstained (The Blom Technique). Stain For Cells, etc.: Similar to Wright Stain.
CONTENTS: Sodium Bicarbonate, Formalin (Neutral), Eosin, Nigrosin, Wright Stain, Methanol, Buffers Sodium Bicarbonate, Formalin (Neutral), Eosin, Nigrosin, Wright Stain, Methanol, Buffers These solutions are made from certified dyes (when applicable).FOR IN VITRO DIAGNOSTIC USE ONLY. SEMEN DILUTING FLUID RECOMMENDED PROCEDURE: Sperm Count: Draw semen up to 0.5 mark (WBC pipette) and dilute to 11.0 mark. Shake well, charge hemacytometer and place in humidified chamber to avoid evaporation if unable to read immediately. Count the number of spermatozoa in 2 sq. mm and add 5 zeros to obtain the number per ml.
CONTENTS: Sodium Bicarbonate, Formalin (Neutral) FOR IN VITRO DIAGNOSTIC USE ONLY. |
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